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cOmplete His-Tag Purification Resin is a sepharose-based, pre-charged, ready-to-use Ni2+ chelate resin for small and large-scale purification of His-tagged. Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions. Protino® Ni-NTA Agarose. Small scale His-Tag fusion protein purification under denaturative conditions. Introduction. High levels of expression of recombinant proteins in a.

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In addition, the His-tag:Ni-NTA affinity chromatography system tolerates high concentrations of urea and guanidine allowing protein purification under. Sodium Chloride · Adding sodium chloride or glycerol can help increase your protein yield by reducing the extent to which other proteins can stick to the resin. The cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC matrix (Immobilized Metal Affinity Chromatography) for the purification of histidine.

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HisLink Protein Purification Systems are designed for purification of polyhistidine (His)-tagged or HQ-tagged proteins directly from culture medium. I am able to express the protein in soluble form but when I purify it using the Ni column, several host proteins purified with my target (expressed) protein. In addition, the His-tag:Ni-NTA affinity chromatography system tolerates high concentrations of urea and guanidine allowing protein purification under.